This experiment is from the official website of Parasitology, School of Medicine, Shandong University.
Operation method
Morphological observation of the Chinese branchiostoma schizothoracinum.
Principle
Chinese branch testis [Clonorchis sinensis (Cobbold, 1875) Looss, 1907] is short for Chinese branch testis, also known as liver fluke, adult parasites in the liver and bile ducts, can cause Chinese branch testis (Clonorchiasis) or liver fluke disease. Life history: Clonorchiasis is parasitized in the liver and bile ducts of humans, cats and dogs. Trichinella-containing eggs are discharged to the intestinal cavity with bile and excreted with feces, and the eggs are ingested by the first intermediate host, such as marsh snails, culicine snails or bean snails, and the trichinellae hatch in the snails through the cytosolic larvae, larvae and caecal larvae, and the caecal larvae escape from the snails, and invade into the second intermediate host of freshwater fish to form cysticerci, and people are infected by eating raw or semi-raw freshwater fish. Cats and dogs are the hosts of this worm. In severe cases, it causes cirrhosis of the liver. Move I. Self-observation Common Problems Fish muscle cysts in the method: only infected with Chinese branch testicular schistosomiasis cysts of carp family freshwater fish (commonly used in fish) in a clean Petri dish, with a small scissors gently scrape off the fish scales, and then use small forceps to tear off the skin of the fish, from the back of the fish to take the muscle a small piece of (mung bean grain size), placed between the two slides and forcefully pressed thin, with a thin line will be the two ends of the slide tied tightly, low-magnification observation of cysticercosis morphology. (The vast majority of the cystic larvae are found on the back of the fish and in the anal region to the base of the caudal fin.) If necessary, it is important to distinguish them from the cystic larvae of other trematodes (e.g., trematodes of the posterior testis of cats, etc.). For more product details, please visit Aladdin Scientific website.
1. Liver fluke egg slide specimen:
Hepatic schistosome eggs are the smallest of the eggs of helminths parasitized in the human body. In the low magnification of its size and shape like a sesame seed. Under high magnification such as the size of watermelon seeds, shaped like an old-fashioned light bulb, the egg shell is thicker, light yellowish brown, one end of the egg has a small cover, the other end has a small spine, the egg has a trichinella. Because the eggs are small, when looking for them under low magnification, the aperture should be narrowed. 
2. Adult liver fluke slide specimens:
Hepatic schistosomes are like sunflower seeds, the body is thin and translucent, the anterior end is thin, the posterior end is obtuse and rounded, the size of the body is about 10～25×3～5 mm, under the microscope, the oral sucking discs are slightly larger than the abdominal sucking discs, and the latter is in the 1/5 of the anterior end of the body. The oral sucker is at the most anterior end of the body, under the pharynx and short esophagus, then bifurcates and extends to the end of the body to form a blind end, with a mouth and no anus. Male and female, two highly branched testes arranged anteriorly and posteriorly in the posterior 1/3 of the body, stained dark red. The branched testes are the main morphological feature of this insect. The ovary is lobulated at the margin and is located before the testis, the large, sac-like fertilization sac is between the testis and the ovary, between the ovary and the ventral sucker is the highly tortuous egg-filled uterus, and the yolk glands are on either side of the middle part of the insect and are stained brownish-yellow. The genital pores are anterior to the ventral sucker.
3. Slide specimen of liver fluke cysts in fish muscle:
Oval or round, containing larvae, excretory sac containing microscopic refractory particles.
II. Demonstration
1. adult liver fluke
(1) Unstained specimen in its natural state, observe the size, shape and color of the liver fluke.
(2) Stained specimens to observe the male and female reproductive system and digestive system in the body. 
2. Intermediate hosts of Schistosoma hepaticum: first intermediate host, culicine snails and second intermediate host, freshwater fish. 
3. Stage of infection: The cysts are parasitized in freshwater fish and are oval in shape, approximately 138 x 115 micrometers in size, with a double-walled capsule and a pronounced excretory capsule.
4. Gross specimen of cat liver parasitized by Schistosoma hepaticum: note the site of adult parasitism in the liver, and the pathological changes in the liver.
5. the morphology of the specimen in the life history preparation stage, trichinae, cystic larvae, larvae, caecilians.