Fluo-4, AM - 5 mM in DMSO , CAS No.273221-67-3

CAS: 273221-67-3 Cat. No.: F196728 Molecular Weight: 1096.94

GRADE & PURITY 5 mM in DMSO

Synonyms

fluo-4 am | Fluo-4AM | AKOS040741728 | HY-101896 | UNII-M7XMU4W82G | YKA22167 | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 | Glycine, N-(4-(6-((acetyloxy)methoxy)-2,7-difluoro-3-oxo-3H-xanthen-9-yl)-2-(2-(2-(bis(2-((acetyloxy)methoxy)-2-oxoethyl)amino)-5-me

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Specifications

Synonyms

fluo-4 am | Fluo-4AM | AKOS040741728 | HY-101896 | UNII-M7XMU4W82G | YKA22167 | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 | Glycine, N-(4-(6-((acetyloxy)methoxy)-2, 7-difluoro-3-oxo-3H-xanthen-9-yl)-2-(2-(2-(bis(2-((acetyloxy)methoxy)-2-oxoethyl)amino)-5-me

Specifications & Purity

5 mM in DMSO

Biochemical and Physiological Mechanisms

Cell-permeable, fluorescent Ca2+indicator (KdCa2+= 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+binding. Excitation/emission λ 494/506 nm.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Names and Identifiers

• Canonical Smiles: CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
• IUPAC Name: acetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-N-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate
• InChIKey: QOMNQGZXFYNBNG-UHFFFAOYSA-N
• INCHI: 1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
• Isomeric SMILES: CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
• Molecular Weight: 1096.94
• Reaxy-Rn: 24143884
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=24143884&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Alpha amino acid esters Aminophenyl ethers Phenoxy compounds Aminotoluenes Dialkylarylamines Aniline and substituted anilines Acylals Alkyl aryl ethers Aryl fluorides Heteroaromatic compounds Cyclic ketones Oxacyclic compounds Acetals Hydrocarbon derivatives Organic oxides Organofluorides Organopnictogen compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Alpha-amino acid ester - Alpha-amino acid or derivatives - Aminophenyl ether - Phenoxy compound - Phenol ether - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Aniline or substituted anilines - Aminotoluene - Alkyl aryl ether - Toluene - Acylal - Benzenoid - Aryl fluoride - Aryl halide - Monocyclic benzene moiety - Heteroaromatic compound - Cyclic ketone - Tertiary amine - Amino acid or derivatives - Carboxylic acid ester - Oxacycle - Acetal - Carboxylic acid derivative - Ether - Organic nitrogen compound - Organohalogen compound - Carbonyl group - Organofluoride - Organonitrogen compound - Organooxygen compound - Organopnictogen compound - Amine - Organic oxygen compound - Organic oxide - Hydrocarbon derivative - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Molecular Weight: 1096.900 g/mol
• XLogP3: 5.500
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 27
• Rotatable Bond Count: 36
• Exact Mass: 1096.28 Da
• Monoisotopic Mass: 1096.28 Da
• Topological Polar Surface Area: 297.000 Ų
• Heavy Atom Count: 78
• Formal Charge: 0
• Complexity: 2220.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Chen Yu, Zhang Yingai, Zhang Yuting, lv Shuguo, Zhang Xu, Mohsen Mohamed, Zhu Xiaoshan, Yin Kai, Zhou Hailong.  (2026)  Polystyrene nanoplastics and benzo(a)pyrene synergistically induce lung fibrosis and inflammation via relaxin signalling in mice.  Communications Biology,      [PMID:] [10.1038/s42003-026-09872-9]