LS 510 Free Acid - BioReagent,Biological Stain,for fluorescence analysis,for microscopy,sterile,Suitable for Immunohistochemistry(IHC),Suitable for Immunofluorescence(IF)

Cat. No.: L1511496

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, Sterile, Suitable for Immunohistochemistry(IHC), Suitable for Immunofluorescence(IF)

Synonyms

LS510 free acid

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Staining, Fluorescence Analysis, IF/ICC, IHC, In situ hybridization (ISH)

Overview

  LS 510 Free Acid is a high-performance fluorescent labeling dye featuring a unique long Stokes shift. With an excitation wavelength of 509 nm and an emission wavelength of 590 nm, its large spectral separation effectively reduces overlap between excitation and emission light, thus minimizing spectral crosstalk at the source. This product is designed to address the insufficient detection accuracy caused by spectral overlap of conventional fluorescent dyes in multiplex experiments. It offers excellent solubility and enhanced signal discrimination in multi-channel labeling due to its long Stokes shift. It also provides balanced performance in fluorescence stability and biocompatibility, meeting the core requirements for precise labeling.

  As a widely used reagent in immunofluorescence assays, LS 510 Free Acid is suitable for cytoskeleton dynamics imaging, immunohistochemistry, fluorescence in situ hybridization, and other multi-labeling applications. It is especially ideal for the analysis of complex samples requiring simultaneous detection of multiple targets. Its long Stokes shift effectively eliminates signal overlap and interference between detection channels, improving the accuracy of multi-target detection.

  Note: Performance is equivalent to Dyomics DY‑510XL.

Applications

Cytoskeleton dynamics imaging; Immunohistochemistry; Fluorescence in situ hybridization (FISH); Multi-target complex sample analysis; Immunofluorescence

Product Features

1. High brightness: Strong emission intensity, brighter than FITC, with higher sensitivity.

2. Excellent photostability: High resistance to photobleaching, ensuring reliable quantitative data and stable results.

3. Buffer stability: The conjugated fluorophore remains fully stable in the pH range of 4 to 9.

4. Low photobleaching and channel crosstalk: Ex/Em: 509/590 nm; long Stokes shift with an Ex‑Em gap of 81 nm.

5. Instrument compatibility: Excitation and emission spectra match filter sets and laser configurations of all major fluorescence detection instruments.

Product Parameters

1. Ex/Em: 509/590 nm

2. Extinction Coefficient (ε): 50000

3. Molecular Weight: 605.14

4. Solubility: Soluble in water, DMSO, DMF

5. Spectrum:

Precautions

1. This product is soluble in DMSO, DMF, and water. Excitation and emission wavelengths may vary slightly in different solvents.

2. Mix thoroughly before use and avoid repeated freeze‑thaw cycles. Upon first use, aliquot the product into small tubes and store at ‑20 °C.

3. Fluorescent dyes are prone to photobleaching; always protect from light during handling to maintain stability.

4. This product is for research use only and must not be stored in residential areas.

5. For your safety and health, please follow the standard laboratory safety regulations of your institution.

Specifications

Synonyms

LS510 free acid

Specifications & Purity

BioReagent, Biological Stain, for fluorescence analysis, for microscopy, sterile, Suitable for Immunohistochemistry(IHC), Suitable for Immunofluorescence(IF)

Stability And Storage

Store at -20℃ long term (60 months). Store in the dark.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy, Sterile, Suitable for Immunofluorescence(IF), Suitable for Immunohistochemistry(IHC)

Chemical and Physical Properties

• Solubility: Soluble in water, DMSO, DMF
• Sensitivity: Light-sensitive