GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.2 mg/mL
ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Pyruvate Dehydrogenase E2 Antibody, Recombinant, could be used for WB, ICC/IF and so on. Application WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Specifications
Product Name
Recombinant Pyruvate Dehydrogenase E2 Antibody - Primary antibody, specific to Pyruvate Dehydrogenase E2, Rabbit IgG
A synthetic peptide derived from human Pyruvate Dehydrogenase E2 (AA 594-608)
Positive Control
WB: HeLa, Ramos, MCF7, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.2 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124049) - Western Blot All lanes: Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124049) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 69 kDa Observed band size: 73 kDa Exposure time: 20.8 s
Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124049) - IF/ICC Immunocytochemistry analysis of Pyruvate Dehydrogenase E2 (green) in HeLa using Recombinant Pyruvate Dehydrogenase E2 Antibody (Ab124049) and DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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