Tris-HCl Antibody Purification Neutralization Buffer (10×) - sterile-filtered, BioReagent

Cat. No.: N1456177

GRADE & PURITY Sterile-filtered, BioReagent

Synonyms

Tris-HCl Neutralization Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Antibody Purification

Overview

Aladdin Neutralization Buffer (10× Tris-HCl, pH 9.0) is a high-concentration, ready-to-dilute solution specifically designed for rapid pH adjustment in antibody purification workflows. This 10X concentrate provides an economical and space-efficient solution for laboratories requiring large volumes of neutralization buffer, ensuring optimal protection of purified antibodies from acid-induced denaturation following low-pH elution

Applications

Key Features

• 10× Concentrated Formula: Cost-effective solution that reduces storage space and shipping costs

• Easy 1:10 Dilution: Simple preparation to 1× working concentration with purified water

• High Buffering Capacity: Effectively neutralizes acidic eluents including pH 2.7-2.8 elution buffers

• Ready-to-Dilute Convenience: Pre-adjusted pH ensures consistent performance after dilution

• Antibody Protection: Prevents prolonged acid exposure that can compromise antibody activity and stability

Applications

• Immediate neutralization of antibodies following Protein A/G/L affinity purification

• Compatible with both manual and automated purification systems

• Suitable for neutralizing eluents from various affinity chromatography methods

• Ideal for research-scale and process development applications

Instructions for Use

1. Dilute the 10× concentrate 1:10 with purified water to obtain 1× working solution

2. Add the 1× working solution to collection tubes (recommended: 1/10 of expected eluent volume)

3. Collect acidic eluate directly into the neutralization buffer

4. Mix gently and verify final pH if necessary

Notes

• Diluted working solution is stable for 4 weeks when stored at 4°C

• For optimal results, use pre-chilled purified water for dilution

• Adjust neutralization volume according to specific elution buffer acidity

• Always neutralize immediately after elution collection

Specifications

Synonyms

Tris-HCl Neutralization Buffer

Specifications & Purity

sterile-filtered, BioReagent

Stability And Storage

Store at 2-8 ℃ long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Names and Identifiers

• PH: pH 9.0