Annealing Buffer for RNA Oligos (10X) - BioReagent, PCR Reagent, Suitable for molecular biology, RNase free, sterile, for DNA and RNA applications, 10X

Cat. No.: A743818
AVAILABLE TO ORDER
GRADE & PURITY Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 10X
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Application
PCR
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
1ml
A743818-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$26.90
5ml
A743818-5ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$93.90
Enter a quantity for the sizes you want to add.
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Why this grade

BioReagent, PCR Reagent, Suitable for molecular biology, RNase free, sterile, for DNA and RNA applications, 10X BioReagent,for DNA and RNA applications,PCR Reagent,RNase free,Sterile,Suitable for molecular biology for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

The Annealing Buffer for RNA Oligos (10X) is a specially formulated buffer designed for the annealing of RNA oligonucleotides. This buffer is particularly useful for annealing complementary RNA oligos synthesized for siRNA applications, enabling the formation of double-stranded RNA. The Annealing Buffer for RNA Oligos (10X) effectively prevents the formation of hairpin structures in RNA oligos, facilitating accurate annealing. The operation is straightforward: simply mix the RNA oligos to be annealed with the Annealing Buffer for RNA Oligos (10X) in the appropriate proportion and place the mixture in a PCR machine. The annealing process can be completed in approximately 60 minutes. The Annealing Buffer for RNA Oligos (10X) has been specially treated to be RNase-free, ensuring that RNA degradation is avoided.

Precautions:

The Annealing Buffer for RNA Oligos (10X) is specifically designed for the annealing of RNA oligos and should not be used for the annealing of DNA oligos.

Since RNA is highly susceptible to degradation, it is essential to strictly follow RNA handling protocols to prevent RNase contamination.

For your safety and health, please wear a lab coat and disposable gloves when operating.

This product is intended for research use only.



Specifications

Specifications & Purity
BioReagent, PCR Reagent, Suitable for molecular biology, RNase free, sterile, for DNA and RNA applications, 10X
Stability And Storage
Store at -20 ℃. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage
Store at -20°C, Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, for DNA and RNA applications, PCR Reagent, RNase free, Sterile, Suitable for molecular biology
Names and Identifiers
PH7.5

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ25F0521259Certificate of AnalysisMar 16, 2026 A743818
ZJ25F0521260Certificate of AnalysisMar 16, 2026 A743818
Documents & Articles
Solution Calculators
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