Diaphorase from Clostridium kluyveri, CAS No.9001-18-7

CAS: 9001-18-7 Cat. No.: D128545 EC Number: 232-587-6

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥30 units/mg dry weight

Bioactivity

≥30 U/mg dry weight

Overview

EPOCROSTM is a reactive polymer with an oxazoline group on the side chain and is used as a cross-linking agent for water-based resins. Among the water-based polymers developed to address environmental issues and the increasing use of water-based products due to VOC regulations and desolventing, the EPOCROSTM WS series is a “water-soluble type” with the following structure.
Features and Properties
Higher reactivity than water-based epoxy, melamine, blocked isocyanate
VOC free (EPOCROS™ WS-300 and EPOCROS™ WS-700)
High crosslinking density with a small amount added
One-pack type with long usage time
Improves water resistance, solvent resistance, heat resistance, and the strength of films, etc.
Adhesion-imparting possible to PET, OPP, PVC, etc.
Fast drying
Low toxicity (Ames Test: Negative, Primary Skin Irritation Test: No irritation)
WS Series Product Lineup
Applications
Nonwoven fabric binders
Pigment printing
Coatings (metals, films, leather)
Paint and coatings, Primers (plastics, building materials, vehicles)
Adhesives

Method
ASSAY for Product Code DILW:
One unit equals a decrease in absorbance of 1.0 per minute at 25°C at pH 7.5 with 2,6-dichlorophenolindophenol as the chromogen.
Reagents
0.2 M Tris⋅HCl buffer, pH 7.5
0.006 M NADH. Prepare fresh daily.
0.0012 M Dichlorophenolindophenol (DCPIP) Prepare fresh daily.
Enzyme
Prepare a 10 mg/ml solution of enzyme in 0.2 M Tris⋅HCl, pH 7.5.
Dilute further immediately before use to give ΔA/min of 0.15-0.20.
Procedure
Adjust spectrophotometer to 600 nm and 25°C.
Pipette into cuvettes as follows:

Mix quickly and measure the decrease in absorbance at 600 nm for 2-3 minutes.
Determine the ΔA/min. from the initial linear portion of the curve. (Use portion of curve from t=0 to t=1 minute; the rate is linear for 1/2 to 1 minute.)

Calculation

Specifications

Product Name

Diaphorase from Clostridium kluyveri, CAS No.9001-18-7

Synonyms

Lipoamide Dehydrogenase | Lipoyl Dehydrogenase

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

EnzymoPure™, ActiBioPure™, Bioactive, High Performance, Native, ≥30 units/mg dry weight

Bioactivity

≥30 U/mg dry weight

CAS

9001-18-7

Enzyme Commission Number

1.6.99.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥30 units/mg dry weight

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Unit definition

1 Unit equals a decrease in absorbance at 600nm of 1.0 per minute at 25°C, pH 7.5.

References

1. Song Wang, Xiewen Wu, Jing Fang, Feng Zhang, Yanli Liu, Hongbo Liu, Yu He, Min Luo, Run Li.  (2023)  Direct Z-Scheme Polymer/Polymer Double-Shell Hollow Nanostructures for Efficient NADH Regeneration and Biocatalytic Artificial Photosynthesis under Visible Light.  ACS Catalysis,      [PMID:] [10.1021/acscatal.2c05722]