ER-Tracker Green

Cat. No.: E745769

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Precautions：

ER-Tracker Green (1mM) will solidify on ice or at lower temperatures such as 4℃ and stick to the bottom, walls or lid of the centrifuge tube. It should be thawed completely in a water bath at 20-25℃ prior to use. For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.The pharmacological activity of glibenclamide may affect some functions of the ER. Variable expression of sulfonylurea receptors in some specific cells may result in non-ER-specific staining.ER-Tracker Green is suitable for staining ER in live cells, but not ER in fixed cells. When cells stained with ER-Tracker Green need to be fixed, fix cells with 4% paraformaldehyde at 37℃ for 2 minutes.ER-Tracker Green stained cells cannot be permeabilized with Triton X-100 which will result in the loss of ER-Tracker Green fluorescent staining.Coverslips and slides are required but not supplied. They can be ordered from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Preparation of ER-Tracker Green working solutiona. Dilute an appropriate amount of ER-Tracker Green (1mM) in the ER-Tracker Green Dilution Buffer at a ratio of 1:1000. For example, mix 1µl of ER-Tracker Green with 1ml of ER-Tracker Green Dilution buffer to obtain the ER-Tracker Green working solution.b.Prewarm the ER-Tracker Green working solution at 37℃ before use.Note: The concentration of ER-Tracker Green in the working solution can be adjusted within the range of 1:1000-1:3000 based on the staining results. To minimize the staining background, a lower concentration of ER-Tracker Green is preferred as long as an acceptable staining result can be obtained.2.Fluorescent labeling of ERa. Remove the cell culture medium and wash cells grown on coverslips with an appropriate amount of solution such as Hanks' Balanced Salt Solution with Ca2+ & Mg2+ (, C0219). For staining of suspension cells, centrifuge to collect cells and refer to the staining method for adherent cells.b.Remove the wash buffer, add the ER-Tracker Green working solution pre-incubated at 37℃, and incubate at 37℃ for 15-30 min.c. Remove the ER-Tracker Green working solution and wash cells 1-2 times with cell culture medium.d.Examine cells by fluorescence microscope or confocal microscope. e. If the ER-Tracker Green stained cells need to be fixed, fix with 4% paraformaldehyde for 2 min at 37℃. After fixation, wash cells 2-3 times with appropriate wash buffer for 5 minutes each, and then counterstain cells or mount cells with antifade mounting medium. Note: ER-Tracker Green stained cells cannot be permeabilized with Triton X-100 which will result in the loss of ER-Tracker Green fluorescent staining.

Specifications

Stability And Storage

Store at -20℃ for up to 6 months. ER-Tracker Green should be protected from light.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.