Experiments on the preparation of tissue specimens from laboratory animals
Experiments on the preparation of tissue specimens from laboratory animals
Rabbits, guinea pigs, rats or mice are usually used and fasted for 24 hours. The head is knocked unconscious to avoid the effects of anesthesia or blood loss on gastrointestinal function. Immediately open the abdominal cavity and remove the stomach, intestines, gallbladder, etc. as required. Remove attached tissues such as tethered membranes or fat. Rapidly place in oxygenated (or 95% O2+5% CO2 ) warmed (37 °C) nutrient solution and clean the lumen of food debris with nutrient solution using a syringe.
Operation method
autopsy
Materials and Instruments
Rabbit, Guinea Pig, White Rat Move Rabbits, guinea pigs, rats or mice are usually used and fasted for 24 hours. The head is knocked unconscious to avoid the effects of anesthesia or blood loss on gastrointestinal function. Immediately open the abdominal cavity and remove the stomach, intestines, gallbladder, etc. as required. Remove attached tissues such as tethered membranes or fat. Rapidly place in oxygenated (or 95% O2 + 5% CO2 ) insulated (37 ℃) nutrient solution and clean the lumen of the tube of food debris with nutrient solution using a syringe. Caveat 1. the operation should be gentle, rinsing specimens should not use high pressure to avoid tissue contracture. 2. The nutrient solution used in the experiment should vary according to different tissues. For the intestinal tube of rabbits, Tirod's solution is preferred, and for gastric muscle and gallbladder slices, Kirschner'sThe gastric muscle and gallbladder slices should be treated with Kirschner's solution. For more product details, please visit Aladdin Scientific website.
Nutrient solution
Syringe Line Bath
1. Preparation of intestinal specimens is usually taken from the duodenum or ileum The duodenum is more excitable and autoregulatory, showing active diastolic activity. The ileum is more sedentary and the baseline of its motility curve is more stable. The specimen used is usually a section of about 1.5 cm. The ends are tied with threads and fixed in the bath. If you want to observe the movement of the longitudinal or circular muscle of the intestinal tube separately, you can dissect the intestinal tube along its longitudinal axis, take a piece of the muscle, and tie the two ends with wires to fix it, so that you can record the activity of the longitudinal muscle. If you want to observe the movement of the circular muscle, the intestinal tube should be cut longitudinally, and then cut in an S-shape, and the two ends should be fixed with wires and pulled to form (Figure 1).
2. Preparation of gallbladder specimens If the gallbladder of a dog is used, a 4 mm wide by 2 cm long slice of the whole muscle can be cut. The gallbladder of the rabbit or guinea pig is smaller and is often removed with the bile duct. The gallbladder of the rabbit can be cut in half along its long axis; that of the guinea pig can be used as a whole gallbladder or half of it for experiments.
3. Specimens of the bile duct are usually prepared from the common bile duct of the dog by removing the adjacent duodenal tissue leaving the papilla and the sphincter of the bile duct undivided.
4. Strips of gastric muscle, as shown in Fig. 2, may be taken according to the alignment of the muscle fibers.
