Identification of IgG purity by polyacrylamide gel electrophoresis
Identification of IgG purity by polyacrylamide gel electrophoresis
Since the concentration of the polyacrylamide gel can be prepared on demand, two electrophoresis systems, the "Continuous System" and the "Discontinuous System," can be formed. The most important feature of the "discontinuous system" is that the resolution of sample separation is greatly improved. The main features of this kind of electrophoresis are: (1) using two different concentrations of gel system; (2) the composition and pH of the buffer solution for preparing the two kinds of gels are different, and also the composition and pH of the electrophoresis buffer in the electrophoresis tank are different from those in the electrophoresis tank. In the experiment, the electrophoresis gel was divided into two layers: the upper layer of the gel was a low-concentration macroporous gel, called concentrated gel or layer-forming gel, and the buffer solution for preparing this layer of the gel was Tris-HCl, pH 6.7; the lower layer of the gel was a high-concentration small-porous gel, called separating gel or electrophoresis gel, and the buffer solution for the formation of the gel was Tris-HCl, pH 8.9, and the buffer solution for electrodes in electrophoresis baths was Tris-glycine, pH 8.3. As can be seen, the gel concentration, gel-forming components, pH and electrophoresis buffer systems are different, forming a discontinuous system.
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