Morphological observation experiment of diphtheria rod-shaped bacillus

Summary

Knowledge of heterochromatic granule staining; knowledge of the morphological staining characteristics of Corynebacterium diphtheriae.

Operation method

Morphological observation of diphtheria rod-shaped bacilli

Principle

Corynebacterium diphtheriae is a gram-positive bacillus with heterochromatic granules and a specific arrangement.

Move

Observe slices of Corynebacterium diphtheriae Gram stained and heterogeneous stained granular stained specimens.

Observation of culture characteristics of Corynebacterium diphtheriae: Observation of colony characteristics of Corynebacterium diphtheriae on Lüscher serum slants and potassium tellurite blood plates.

Heterogeneous staining of particles: Neisser's staining method

Neisser staining method: Take the culture of Corynebacterium diphtheriae cultured in Lüscher's serum slant and mix the plate with physiological saline. Add the mixture of the first and second solution of Neisser's stain to the flame-fixed specimen, stain for 1-3 minutes, wash with water, and then stain with the third solution for half a minute, pour off the stain, and then dry for microscopic examination. The body of Corynebacterium diphtheriae was yellow-brown, and the heterochromatic particles were blue-black.

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