Intrauterine microelectroporation for efficient gene transfer in mouse embryos
Intrauterine microelectroporation for efficient gene transfer in mouse embryos
The introduction of genes into the embryo has been successfully achieved by a new technique of electroporation of the chicken embryo. In contrast, since the mammalian embryo is enclosed by the mother's uterus, genetic manipulation of the mammalian embryo in a specific region of the uterus during most stages of development has been difficult, if not impossible, using traditional methods. To address this difficulty, we have developed the intrauterine micro-electroporation technique, which uses fine tungsten and platinum wires placed into the cerebral ventricle of the mouse embryo to introduce DNA into the mouse embryo. This transient effect on brain development was detected from the embryonic stage to maturity, although the ectopic expression of the gene lasted only a few days. Author: T. Friedman et al, Translator: Jingwei et al. This experiment is from "Gene Transfer".
Operation method
Electroporation of mouse embryos in utero Move Electroporation of mouse embryos in utero Materials reagents Ethanol (50 %) Solid green F C F protein staining reagent (Sigm a-A ld rich ) Phosphate Buffer (P B S) Pregnant mice (E 10.5 or longer) Sodium pentobarbital Instrumentation Stainless steel small surgical clips For more product details, please visit Aladdin Scientific website.
DNA, solution to be introduced (l ug/ul)
