Thermophilus Vitamin-Mineral Stock 100X (TYE Media, Castenholz Media) (Powder)

Directions per Liter:

Dissolve 4.052g per liter of ddH2O, stirring gently until completely solubilized. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: The 100X liquid medium forms precipitates at pH values greater than 5. If higher pH is required, it is advisable to prepare a 2X solution from the stock 100X solution, and adjust this solution to the desired pH. Filter-sterilize using a 0.22 micron membrane filter. Adjust the volume accordingly for use with Thermophilus Growh Medium Base Broth.

Minerals	g/liter
Boric Acid	0.05
Calcium Chloride	0.66
Cobalt Nitrate	0.005
Copper Sulfate	0.015
Ferric Chloride	0.5
Magnesium Chloride	2.5
Manganese Sulfate	0.2
Nickel Chloride	0.002
Sodium Molybdate	0.0025
Zinc Sulfate	0.05
Vitamins
p-Aminobenzoate	0.008
Biotin	0.001
Choline Bromide	0.005
Cyanocobalamin	0.00004
Folic Acid	0.001
myo-Inositol	0.002
Nicotinamide	0.008
Monosodium Orotic Acid	0.002
D-Pantothenic Acid•Ca	0.008
Pyridoxine Hydrochloride	0.008
Riboflavin	0.004
Spermidine	0.01
Thiamine Hydrochloride	0.01
	Total: 4.052g/Liter

Storage and Stability:

Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.