GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.≥20,000,000 units/g protein, expressed in E. coli, aqueous glycerol solution
Recombinant, ≥20,000,000 units/g protein, expressed in E. coli, aqueous glycerol solution Recombinant for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.
Application:
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Specifications
Product Name
β-Glucuronidase from Escherichia coli, CAS No.9001-45-0
Grade
Recombinant
Specifications & Purity
Recombinant, ≥20, 000, 000 units/g protein, expressed in E. coli, aqueous glycerol solution
1.Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi. (2020) Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis. STEROIDS, [PMID:32603755][10.1016/j.steroids.2020.108691]
2.Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu. (2018) Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection. TALANTA, [PMID:30172485][10.1016/j.talanta.2018.07.073]
Solution Calculators
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Reviews
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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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