NeutrAvidin Protein (HRP)

Cat. No.: rp188518

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Bioactivity

Binding Biotin

Overview

NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding.
Features include:
• Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin
• Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin
• No RYD recognition sequence—no known off-target binding domains like streptavidin
• Affordable—significantly less expensive than streptavidin
NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.

Specifications

Product Name

NeutrAvidin Protein (HRP)

Synonyms

Nuetral Avidin | NeutrAvidin | NeutrAvidin - Horseradish Peroxidase Conjugated

Grade

ExactAb™, Validated

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Bioactivity

Binding Biotin

Conjugation

HRP

Predicted molecular weight

60kDa

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Western Blot: NeutrAvidin Protein (HRP) (rp188518)

NeutrAvidin Protein (HRP) (rp188518) - Western Blot
All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: NeutrAvidin Protein (HRP) (rp188518) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 3.0 s

Application

• Application: Dilution info
• WB:

  1/5000-1/80000;

• ELISA:

  1:5000-1:50000;