GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1 μL/test
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Host species
Mouse
Specificity
Human CD3ε
Immunogen
Human thymocytes
Positive Control
Flow Cytometry:Human peripheral blood lymphocytes
Conjugation
PerCP-Cy5.5
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
690nm
Ex/Em(nm)
Ex:482nm;Em:690nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
1 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (PerCP-Cy5.5) (Ab230568) at 1μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PerCP-Cy5.5) (Ab190081).
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (PerCP-Cy5.5) (Ab230568) at 1μL/test for 1 hour at 4°C. Blue - Mouse IgG (PerCP-Cy5.5) (Ab190081). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
1μl/Test
For flow cytometric staining, the suggested use of this reagent is 1 µL per million cells in 100 µL staining volume or 1 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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