GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Cdc34 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/2000 ICC/IF: 1/50-1/200 Protein Function： Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase throught its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
Specifications
Product Name
Recombinant Cdc34 Antibody - Primary antibody, specific to Cdc34, Rabbit IgG
A synthetic peptide derived from human Cdc34 (AA 2-236).
Positive Control
WB: Jurkat, Hep G2, K562, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Cdc34 Antibody (Ab095618) - Western Blot All lanes: Recombinant Cdc34 Antibody (Ab095618) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 31 kDa Exposure time: 42.7 s
Recombinant Cdc34 Antibody (Ab095618) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618).
Recombinant Cdc34 Antibody (Ab095618) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618) at 1/25 dilution.
Recombinant Cdc34 Antibody (Ab095618) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Cdc34 Antibody (Ab095618) at 1/100 dilution.
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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