Recombinant Human Gremlin 1 Protein, ＞98%(SDS-PAGE)

Cat. No.: rp146650

GRADE & PURITY Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, ≥98%(SDS-PAGE)

Expression System

E. coli

Accession #

O60565

Protein Tag

N-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Gremlin 1 is an inhibitor in the TGF-beta signaling pathway. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. Measured by its binding ability in a functional ELISA. Immobilized Human BMP-7 at 2 μg/mL can bind Recombinant Human Gremlin 1 Protein (rp146650) with the EC50 of 0.10 μg/mL.

Overview

Background:
Gremlin, also known as Increased in High Glucose protein 2 (IHG-2) and Down-regulated in Mos-transformed cells protein (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins . Human Gremlin is synthesized as a 184 amino acid (aa) precursor that contains a 24 aa signal sequence and a 160 aa mature region (SwissProt # O60565). The mature region contains one potential site for N-linked glycosylation (Asn 42), a cysteine-rich region, and a cysteine-knot motif (aa 94184) whose structure is shared by members of the TGF-beta superfamily. Post-translational modifications include glycosylation and phosphorylation (3). Gremlin exists in both secreted and membrane-associated forms. There are two isoforms for human Gremlin. Isoform 1 is the standard protein, and in isoform 2, there is a deletion of aa 3979. Human Gremlin shares 99% and 86% aa sequence identity with mouse and chick Gremlin, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle.
Funtion:
Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.
Tissue specificity：
Highly expressed in small intestine, fetal brain and colon. Expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. In subjects with HMPS1, by contrast, GREM1 is expressed, not only in basal ISEMFs, but also at very high levels in epithelial cells (predominantly colonocytes), with expression extending most of the way up the sides of the crypt. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts.

Specifications

Product Name

Recombinant Human Gremlin 1 Protein, ＞98%(SDS-PAGE)

Synonyms

Cell proliferation-inducing gene 2 protein | CKTSF1B1gremlin 1, cysteine knot superfamily, homolog | Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis) | DAN domain family member 2 | DAND2 | DAND2GR

Grade

ActiBioPure™, Azide Free, Bioactive, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥98%(SDS-PAGE)

Purity

＞98%(SDS-PAGE)

Bioactivity

Gremlin 1 is an inhibitor in the TGF-beta signaling pathway. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. Measured by its binding ability in a functional ELISA. Immobilized Human BMP-7 at 2 μg/mL can bind Recombinant Human Gremlin 1 Protein (rp146650) with the EC50 of 0.10 μg/mL.

Endotoxin Concentration

<1.0 EU/μg

Expression System

E. coli

Species

Human

Amino Acids

26-184 aa

Sequence

KKGSQGAIPPPDKAQHNDSEQTQSPQQPGSRNRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD

Protein Tag

N-His

N-terminal Sequence

K

Accession #

O60565

Predicted molecular weight

24.9 KDa

SDS-PAGE

23.2 kDa, under reducing conditions; 23.2 kDa, under non-reducing conditions.

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ for one month. Aliquot and store at -80℃ for 12 months.

Images

Protein Bioactivity: Recombinant Human Gremlin 1 Protein (rp146650)

Recombinant Human Gremlin 1 Protein (rp146650) - Protein Bioactivity
Gremlin 1 is an inhibitor in the TGF-beta signaling pathway. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers.
Measured by its binding ability in a functional ELISA. Immobilized Human BMP-7 at 2 μg/mL can bind Recombinant Human Gremlin 1 Protein (rp146650) with the EC50 of 0.10 μg/mL.

SDS-PAGE: Recombinant Human Gremlin 1 Protein (rp146650)

Recombinant Human Gremlin 1 Protein (rp146650) - SDS-PAGE
3 μg/lane of Recombinant Human Gremlin 1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 23.2 kDa.

Genetic information

• Alternate Names: Cell proliferation-inducing gene 2 protein | CKTSF1B1gremlin 1, cysteine knot superfamily, homolog | Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis) | DAN domain family member 2 | DAND2 | DAND2GR
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