GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.See COA
Recombinant, ExactAb™, KD Validation, Validated, See COA ExactAb™,KD Validation,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human MEK3/MEK6 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/1000-1/2000 IHC: 1/100-1/200
Protein Function:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Specifications
Product Name
Recombinant MEK3/MEK6 Antibody - Primary antibody, specific to MAP2K3; MAP2K6, Rabbit IgG
Recombinant, ExactAb™, KD Validation, Validated, See COA
Host species
Rabbit
Specificity
MAP2K3; MAP2K6
Immunogen
A synthetic peptide derived from human MEK3 (AA 300-347)
Positive Control
WB: Mouse heart, Rat heart, HeLa, MCF7, NIH/3T3 and C6 lysates. IHC: Mouse spleen cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, KD Validation, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MEK3/MEK6 Antibody (Ab114875) - Western Blot All lanes: Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 36, 38 kDa Recombinant MEK3/MEK6 Antibody (Ab114875) recognizes two isoforms of MEK3/MEK6. Exposure time: 30.0 s
Recombinant MEK3/MEK6 Antibody (Ab114875) - Western Blot All lanes: Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 36, 38 kDa Recombinant MEK3/MEK6 Antibody (Ab114875) recognizes two isoforms of MEK3/MEK6. Exposure time: 30.0 s
Recombinant MEK3/MEK6 Antibody (Ab114875) - Western Blot All lanes: Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 38 kDa Exposure time: 30.0 s
Recombinant MEK3/MEK6 Antibody (Ab114875) - IHC Immunohistochemical analysis of paraffin-embedded mouse spleen, using Recombinant MEK3/MEK6 Antibody (Ab114875).
Recombinant MEK3/MEK6 Antibody (Ab114875) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant MEK3/MEK6 Antibody (Ab114875).
Recombinant MEK3/MEK6 Antibody (Ab114875) - IF/ICC IF analysis of Recombinant MEK3/MEK6 Antibody (Ab114875) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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