GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human MiTF Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Specifications
Product Name
Recombinant MiTF Antibody - Primary antibody, specific to MITF, Rabbit IgG
Synonyms
bHLHe32 | Class E basic helix-loop-helix protein 32 | BHLHE32 antibody | Class E basic helix-loop-helix protein 32 antibody | CMM8 antibody | Homolog of mouse microphthalmiaantibody | Mi antibody | Microphthalmia associated transcription factor antibody |
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived
Host species
Rabbit
Specificity
MITF
Immunogen
A synthetic peptide derived from human MiTF (AA 350-526)
Positive Control
WB: HeLa, HEK293, A375, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant MiTF Antibody (Ab115427) - Western Blot All lanes: Recombinant MiTF Antibody (Ab115427) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa Observed band size: 50 - 70 kDa The molecular weight observed is consistent with what has been described in the literature (PMID: 17182576). Exposure time: 12.2 s
Recombinant MiTF Antibody (Ab115427) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant MiTF Antibody (Ab115427) at 1/50 dilution.
Recombinant MiTF Antibody (Ab115427) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant MiTF Antibody (Ab115427) at 1/50 dilution.
Application
Application
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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