Recombinant NEDD8 Antibody - Primary antibody, specific to NEDD8, Rabbit IgG

Cat. No.: Ab117492
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. See COA
Application
WB
Host species
Rabbit
Reactive species
Human, Mouse, Rat
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab117492-10μl
1
$99.90
50μl
Ab117492-50μl
1
$259.90
100μl
Ab117492-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab117492-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human NEDD8 Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name
Recombinant NEDD8 Antibody - Primary antibody, specific to NEDD8, Rabbit IgG
Synonyms
NEDD-8 | Neddylin | Neural precursor cell expressed developmentally down-regulated protein 8 | Ubiquitin-like protein Nedd8 | FLJ43224 antibody | MGC104393 antibody | MGC125896 antibody | MGC125897 antibody | NED8 antibody | NEDD 8 antibody | NEDD-8 antib
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins
Host species
Rabbit
Specificity
NEDD8
Immunogen
A synthetic peptide derived from human NEDD8 (AA 1-40)
Positive Control
WB: K562, Jurkat, HeLa, THP-1, HEK293, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant NEDD8 Antibody (Ab117492) - Western Blot
All lanes: Recombinant NEDD8 Antibody (Ab117492) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 9 kDa
Observed band size: 10, 89 kDa
The band at 10 kDa represents free NEDD8 protein, while the band at 89 kDa corresponds to Cullin proteins that have undergone Neddylation modification. NEDD8 is a small protein that typically modifies Cullin proteins through covalent binding, resulting in the formation of Neddylated Cullins. This modification plays an important role in processes such as cell cycle regulation and protein degradation.
Exposure time: 4.2 s

Application
ApplicationDilution info
WB

1/1000-1/2000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
NEDD8 Tchem NEDD8 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot NumberCertificate TypeDateItem
ZJ25F0116661Certificate of AnalysisJan 07, 2025 Ab117492
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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