GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human BEGAIN Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application WB: 1/500-1/1000 IHC: 1/100 - 1/200 IF/ICC :1/100 - 1/500 Protein Function May sustain the structure of the postsynaptic density (PSD).
Specifications
Product Name
BEGAIN Antibody - Primary antibody, specific to BEGAIN, Rabbit IgG
Synonyms
BEGAIN | BEGIN | Brain enriched guanylate kinase associated protein | Brain-enriched guanylate kinase-associated homolog (rat) | KIAA1446 | SAP90/PSD95 Associated Proteins | SAPAP | BEGIN_HUMAN | Begain antibody | BEGIN antibody | BEGIN_HUMAN antibody | B
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species
Rabbit
Specificity
BEGAIN
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BEGAIN
Positive Control
WB: HuT 78 cell lysate. IHC: Human breast cancer tissue. IF/ICC: HeLa cells
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
BEGAIN Antibody (Ab090930) - Western Blot All lanes: BEGAIN Antibody (Ab090930) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 65 kDa Observed band size: 60 kDa Exposure time: 2.0 s
BEGAIN Antibody (Ab090930) - IHC Immunohistochemical analysis of BEGAIN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with BEGAIN Antibody (Ab090930) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
BEGAIN Antibody (Ab090930) - IF/ICC Immunofluorescent analysis of BEGAIN staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% TritonX-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with BEGAIN Antibody (Ab090930) in 3% BSA-PBS and incubated over night at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody(red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Application
Application
Dilution info
IF/ICC
1/100-1/500
WB
1/500-1/1000
IHC
1/100-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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