Bilirubin Oxidase (BOD), CAS No.80619-01-8

CAS: 80619-01-8 Cat. No.: B774050 EC Number: 838-551-1

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥50 U/mg protein

Accession #

P07788

Bioactivity

≥50 U/mg protein; Unit definition: One unit will convert one micromole of bilirubin to biliverdin per min at pH 8.0 at 25℃.

Overview

Source: Microorganism 
Isoelectric point: 5.2 
Michaelis constant: 1.2×10^-4 M (Bilirubin) 
Optimum pH: 7.5                     Fig. 1
Optimum temperature: 25℃~60℃                     Fig. 3
pH Stability: 7.5~11.0 (25℃, 18hr)                     Fig. 2
Thermal stability: <50℃ (pH 7.0, 30min)                     Fig. 4
Inhibitors: BME,NaN3 
Effect of various chemicals:                     Table 1 

Reaction:

Specifications

Product Name

Bilirubin Oxidase (BOD), CAS No.80619-01-8

Synonyms

bilirubin oxidase | cotA

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥50 U/mg protein

Bioactivity

≥50 U/mg protein; Unit definition: One unit will convert one micromole of bilirubin to biliverdin per min at pH 8.0 at 25℃.

Accession #

P07788

Predicted molecular weight

67 kDa (SDS-PAGE)

CAS

80619-01-8

Enzyme Commission Number

EC 1.3.3.5

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥50 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20 (5 years). Upon delivery aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

References

1. Jing Xu, Yujin Li, Yinbing Liu, Xu Wu, Ke-jing Huang.  (2023)  Ultra-sensitive self-powered sensor based on the hollow Rubik's cube substrate and multiple signal amplification strategy for real-time heavy metal detection.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2023.134377]

2. Zengqiang Liang, Yuanqiang Sun, Huajin Zeng, Haimei Qin, Ran Yang, Lingbo Qu, Ke Zhang, Zhaohui Li.  (2023)  Broad-Specificity Screening of Pyrethroids Enabled by the Catalytic Function of Human Serum Albumin on Coumarin Hydrolysis.  ANALYTICAL CHEMISTRY,      [PMID:36952638] [10.1021/acs.analchem.2c05556]

3. Jinyue Shi, Yu Lin, Weiling Qin, Mingxiang Li, Yuyi Zhou, Yeyu Wu, Hu Luo, Ke-Jing Huang, Xuecai Tan.  (2022)  Superior performance of a graphdiyne self-powered biosensor with exonuclease III-assisted signal amplification for sensitive detection of microRNAs.  ANALYST,  147  (22): (4991-4999).  [PMID:36239165] [10.1039/D2AN01384C]

4. Fu-Ting Wang, Hu Luo, Yang-Yang Hou, Yu Ya, Ke-Jing Huang, Shu-Peng Li, Lan-Rui Wang, Ya-Lin Song, Xuecai Tan.  (2022)  Dual microRNAs-driven enzyme biofuel cells: Visualization biosensing system with capacitor assistance output signal amplification.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2022.131970]

5. Xiaoke Zheng, Hanyu Yang, Lan Qin, Siqian Wang, Lei Xie, Lu Yang, Weimin Kong, Liang Zhu, Li Liu, Xiaodong Liu.  (2021)  Bile Duct Ligation Upregulates Expression and Function of L-Amino Acid Transporter 1 at Blood–Brain Barrier of Rats via Activation of Aryl Hydrocarbon Receptor by Bilirubin.  Biomedicines,  9  (10): (1320).  [PMID:34680437] [10.3390/biomedicines9101320]

6. Fu-Ting Wang, Yi-Han Wang, Jing Xu, Ke-Jing Huang.  (2020)  A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell.  Journal of Materials Chemistry B,  8  (7): (1389-1395).  [PMID:31971223] [10.1039/C9TB02574J]

7. Jing Xu, Xinqi Luo, Yujin Li, Yinbing Liu, Jianqi Lu, Xi Lin, Ke-Jing Huang.  (2024)  A self-powered sensor based on hollow bimetallic sulfide and double-strand TSDR amplification achieves sensitive miRNA-21 detection.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136487]

8. Jing Xu, Yinbing liu, Xinqi Luo, Yujin Li, Yiqing Xing, Ke-Jing Huang.  (2024)  Visual self-powered platform for ultrasensitive heavy metal detection designed on graphdiyne/graphene heterojunction and DNAzyme-triggered DNA circuit strategy.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2024.150151]