GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human COL25A1 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Specifications
Product Name
COL25A1 Antibody - Primary antibody, specific to COL25A1, Rabbit IgG
Synonyms
Alzheimer disease amyloid associated protein antibodyAlzheimer disease amyloid-associated protein antibody | AMY antibody | CLAC antibody | CLAC P antibody | CLAC precursor antibody | CLAC-P antibody | Col25a1 antibody | COL25A1 protein antibody | Collage
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms
Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.
Post-translational: Undergoes proteolytic cleavage by furin protease to yield
Host species
Rabbit
Specificity
COL25A1
Immunogen
A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 25 alpha 1.
Positive Control
WB: HEK293 and HeLa cell lysates. IHC: Human brain tissue. IF/ICC: Hep G2 cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
COL25A1 Antibody (Ab191534) - Western Blot All lanes: COL25A1 Antibody (Ab191534) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 64 kDa Observed band size: 99 kDa Exposure time: 4.9 s
COL25A1 Antibody (Ab191534) - IHC Immunohistochemical analysis of Collagen 25 alpha 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with COL25A1 Antibody (Ab191534) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
COL25A1 Antibody (Ab191534) - IF/ICC Immunofluorescent analysis of Collagen 25 alpha 1 staining in Hep G2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the COL25A1 Antibody (Ab191534) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Application
Application
Dilution info
IF/ICC
1/50 - 1/200
IHC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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