Collagen VII Antibody - Primary antibody, specific to Collagen VII, Rabbit IgG

Cat. No.: Ab097220

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human Collagen VII Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/200
ICC/IF: 1/100-1/500
Protein Function
Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Specifications

Product Name

Collagen VII Antibody - Primary antibody, specific to Collagen VII, Rabbit IgG

Synonyms

Alpha 1 type VII collagen antibody | CO7A1_HUMAN antibody | Col7a1 antibody | Collagen 7 antibody | Collagen alpha 1(VII) chain antibody | Collagen alpha-1(VII) chain antibody | Collagen type VII alpha 1 antibody | Collagen VII alpha 1 polypeptide antibod

Specifications & Purity

Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Host species

Rabbit

Specificity

Collagen VII

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 7 alpha 1.

Positive Control

WB: A431 cell lysate. IHC: Human lung cancer tissue. IF/ICC: COS7 cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Collagen VII Antibody (Ab097220)

Collagen VII Antibody (Ab097220) - Western Blot
All lanes: Collagen VII Antibody (Ab097220) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 295 kDa
Observed band size: 290 kDa
The bands beneath the target band (290 kDa) are likely to be degraded target fragments.
Exposure time: 200.0 s

IF/ICC: Collagen VII Antibody (Ab097220)

Collagen VII Antibody (Ab097220) - IF/ICC
Immunofluorescent analysis of Collagen 7 alpha 1 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1%  Triton X-100 in TBS for 5-10 minutes and blocked with 3%  BSA-PBS for 30 minutes at room temperature. Cells were probed with Collagen VII Antibody (Ab097220) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IHC: Collagen VII Antibody (Ab097220)

Collagen VII Antibody (Ab097220) - IHC
Immunohistochemical analysis of Collagen 7 alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Collagen VII Antibody (Ab097220) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

• Application: Dilution info
• IHC:

  1/50-1/200;

• WB:

  1/500-1/1000 ;

• ICC/IF:

  1/100-1/500

Associated Targets(Human)

COL7A1 Tbio Collagen alpha-1(VII) chain (0 Activities)

Discover Target: COL7A1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)