IPTG – 100mM (100X) Stock Solution

Instructions

1. Weigh 0.238 g of IPTG (IPTG, Aladdin Catalog # I274316, I104812 [CAS 367-93-1, mw. = 238.3g/mol]).

2. Add 10 ml sterile H2O. Dissolve completely.

3. Prewet a 0.22 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water.

4. Sterilize IPTG Stock through the prepared 0.22 µm syringe filter.

5. Store in 1 ml aliquots at -20°C for up to 1 year.

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