Acute erythroleukemia (M6) test
Acute erythroleukemia (M6) test
Erythremia is a malignant proliferation of cells of the red lineage. Erythroleukemia (erthrdeukemia) or Di-Gugliemo syndrome is a malignant proliferation of cells of both the red and leukocyte lineage. Leukemia stage: abnormal proliferation of both erythroid and leukocyte lineages. 3. Leukemia stage: transformation of erythroleukemia into a pure and fragile acute granulomatous or acute granulomatous monolithic disease. This experiment is from Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory testing majors.
Operation method
Acute red leukemia (M6 type) experimental
Principle
Erythremia is a malignant proliferation of cells of the red lineage. Erythroleukemia (erthrdeukemia) or Di-Gugliemo syndrome is a malignant proliferation of cells of both the red and leukocyte lineage. Leukemia stage: abnormal proliferation of both erythroid and leukocyte lineages. 3. Leukemia stage: transformation of erythroleukemia into a pure and fragile acute or acute monocytic disease. 4. leukemia stage: transformation of erythroleukemia into a pure and fragile acute or acute monocytic disease. Move Hematology: For more product details, please visit Aladdin Scientific website.
1. Erythrocytic stage: anemia varies in severity, hemoglobin is often 20-10g/L. The degree of anemia increases with the progression of the disease, and it can be seen that all stages of juvenile red blood cells, predominantly proerythrocytes and early juvenile red blood cells, juvenile red blood cells have a peculiar morphology and have a megaloblastoid affected, the reticulocytes are mildly increased, and in a few cases, they are normal or low. The white blood cell count is lower than normal, with the progression of the disease white blood cell count can be increased, platelets are often reduced.
2. erythroleukemia stage: hemoglobin and red blood cell count mostly from moderate to severe reduction, blood film can be seen in basophilic dots color target-shaped and heterogeneous red blood cells, and can be seen in various stages of young red blood cells, in order to the middle and late young red blood cells are more and more. Late juvenile erythrocytes are prevalent and have an abnormal morphology. White blood cell count is generally low, a few cases of normal or elevated, can see the original pull and early juvenile granulocytes, with the development of the disease, juvenile granulocytes gradually increase, some cases of late development of acute myeloid leukemia, its blood image also changed, this time, the juvenile red blood cells gradually reduced platelet reduction is obvious, can be seen in the deformed platelets.
Bone marrow image:
1. Erythroleukemia stage: the bone marrow proliferation is extremely active or obviously active, the nucleated cells in the red lineage hyperplasia is dominant, most of the cases are more than 50% of the granulocyte-red ratio inversion of the original red and early juvenile red is common. Often there is a lack of young red blood cell stage such as "erythrocytic cleft" phenomenon, and there are often morphological abnormalities, such as giant-like changes, nuclear fragmentation, multinucleated and giant nuclei, etc., filamentous schizophrenic cells increase if the heterozygous erythrocytes more than 10%, and bone marrow red blood cells accounted for 30% of the diagnostic significance.
2. erythroleukemia stage: the bone marrow proliferation is extremely active, and both red lineage and granulocyte (or mononuclear lineage) cells show malignant proliferation at the same time. Most of the cases are characterized by middle and late erythrocytes. Most of the cases are dominated by middle and late erythrocytes, followed by primary red and early erythrocytes, but some cases have more primary red and early erythrocytes than middle and young erythrocytes. However, in some cases, there are more early erythrocytes than middle and late erythrocytes. Late juvenile erythrocytes, the morphology of juvenile erythrocytes in this disease is characterized by megaloblastic changes (huge cytoplasmic chromatin detailed cytoplasmic rich, often protruding) and parabasal erythrocytic changes (irregular nuclear shape, nuclear depression. Twisted. Dinucleated. Multinucleated. The nuclei are fragmented and macronuclei are evident. In addition, there is a nucleoplasmic imbalance in the development of young erythrocytes and uneven cell size at the same stage. The leukocyte system is obviously proliferating, the primary granulocytes (or primary monocytes + young monocytes) cells are dominant, more than 30% (ANC) in some of the primitive and naïve cells can be seen in the Auer's bodies, the white blood cells are also megaloblasts and abnormal morphology of the alterations, the filamentous schistocytes, megakaryocytes are common, a significant reduction in the number of megakaryoblasts, the disease and megaloblastic anemia should be differentiated.