Experiment for the determination of serum chloride by the colorimetric method of mercury thioglycolate

Summary

Chloride in the specimen interacts with mercury thiocyanate to produce mercury chloride that is difficult to dissociate and releases a considerable amount of thiocyanate ions, which combine with iron ions in the reagent to produce orange-red ferric thiocyanate, the depth of which is directly proportional to the content of chlorides. This experiment is from Mudanjiang Medical College undergraduate 5-year laboratory guide for testing majors.

Operation method

Experiment for the determination of serum chloride by the colorimetric method of mercury thiocyanate

Principle

Chloride in the specimen interacts with mercury thiocyanate to produce mercuric chloride, which is difficult to dissociate, and releases a considerable amount of thiocyanate ions, which combine with the iron ions in the reagent to produce an orange-red ferric thiocyanate, the depth of which is directly proportional to the amount of chloride.

Materials and Instruments

Saturated mercury thiocyanate solution Mercury nitrate solution Chloride standard solution

Move

I. Experimental reagents: saturated mercuric thiocyanate solution, mercuric nitrate solution, 6%, color development application solution, chloride standard solution.

Second, the experimental operation:

Mix well, set at room temperature for 10 minutes, adjust the zero with the reagent blank tube, compare the color at 450nm and read the optical density of each tube.

III.Calculation:

Chloride mmol/L = (absorbance of measurement tube - absorbance of measurement blank tube)/ absorbance of standard tube × 100

Reference value: Chloride: for 96-108mmol/L

Caveat

1. This method is not specific for chloride ion, and some other halogen elements are as follows: F- Br- and its color reaction, but in normal human blood, the content of the above elements is relatively low, so it can be ignored.

2. If the chloride content in serum samples is more than 125mmol/L or less than 75mmol/L, the serum should be diluted 1:l with distilled water or the serum dosage should be increased by O.5 times, then the test should be carried out, and the result should be multiplied by the number of times of dilution or divided by the number of times of water added to the sample.

3. color temperature: should not be less than 20 ℃ room temperature is too low, still produce turbidity, affecting colorimetric.

4. Each batch of specimen determination, should be measured at the same time the normal or abnormal value of the quality control serum, the resulting value should be within the permissible error, otherwise the reason should be found.

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