Experimental determination of fecal fertilizers by boiling with high mercury chloride

Summary

If fecal urocholine (fecal cholestrol) is present in the feces, add high mercury chloride to produce a red color compound. This experiment is derived from the Mudanjiang Medical College undergraduate 5-year clinical laboratory guide.

Operation method

Experimental determination of fecal fertilizers by boiling with high mercury chloride

Principle

If urobilin (fecal cholestrol) is present in the feces, the addition of high mercury chloride produces a red compound.

Materials and Instruments

Chlorinated high-mercury saturated liquid

Move

Add 1 small piece of fresh stool 0.5g NaCI, 3-4ml of saturated solution of high mercury chloride to the evaporator and mix thoroughly. Heat and boil for 3 minutes to observe the result.

The results of the experiment to determine: if the fecal bilirubin red, containing fecal cholagens oxidized to bilirubin green.

Caveat

1. the result is not easy to determine, should pay attention to the color of the stool, if necessary, with saline instead of reagent blank control.

2. Stool specimens must be fresh. If it contains fat droplets first extracted with ether.

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