Modeling experiments in an animal model of acute obstructive septic cholangitis

Summary

Acute obstructive suppurative cholangitis (acute obstructive suppurative cholangitis) is the severe stage of acute cholangitis, also known as acute severe cholangitis. The pathogenesis of this disease is based on biliary obstruction and bacterial infection, and the disease develops rapidly, which is life-threatening in severe cases. Therefore, it is necessary to establish an animal model of acute obstructive suppurative cholangitis for the study of acute obstructive suppurative cholangitis.

Principle

The basic principle of animal models of acute obstructive suppurative cholangitis is to artificially ligate the left hepatic duct and inject Escherichia coli bacteria to cause septic infection, forming animal models of acute obstructive suppurative cholangitis.

Operation method

Modeling experiments in an animal model of acute obstructive septic cholangitis

Principle

The basic principle of animal models of acute obstructive suppurative cholangitis is to artificially ligate the left hepatic duct and inject Escherichia coli bacteria to cause septic infection, forming animal models of acute obstructive suppurative cholangitis.

Materials and Instruments

Equipment:
Blood plate, syringe, operating microscope, 4-gauge needle injection tip, 5-0 silk thread, Wistar mice, etc.
Reagents:
① saline; ② sodium pentobarbital
Sodium pentobarbital

Move

The basic procedure for modeling acute obstructive septic cholangitis can be divided into the following steps:

A Before the experiment, Escherichia coli type 0111B4 (Beijing Institute of Biology) was inoculated on a blood plate, incubated at 37 ℃ for 24 hours, rinsed with saline to make a final concentration of 5x10 cfu/mL bacterial solution, and stored at 4 ℃.

B Wistar mice were fasted for 12 hours before surgery, anesthetized by intraperitoneal injection of 1% sodium pentobarbital (30 mg/kg), and then incised in the middle of the body. The common bile duct and the left, middle and right hepatic ducts were exposed and freed under a 10× operating microscope, and the left hepatic duct was fully freed, and the left hepatic duct was ligated by a 5-0 wire close to the confluence of the hepatic ducts, and then punctured by a 4-gauge needle with the injection tip, and then slowly injected into Escherichia coli (0.5 mL/kg). 0.5 mL/kg) and proximally ligated the left hepatic duct, the cumulative morbidity and mortality rates at each time point were 0%, 4.1%, 10.0% and 16.7% at 24 hours, 48 hours, 72 hours and 92 hours, respectively.

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