GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Validated, ExactAb™, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human HIF-2-alpha Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
HIF-2-alpha Antibody - Primary antibody, specific to HIF-2-alpha, Rabbit IgG
Synonyms
Basic helix loop helix PAS protein MOP2 antibody | Basic-helix-loop-helix-PAS protein MOP2 antibody | bHLHe73 antibody | Class E basic helix-loop-helix protein 73 antibody | ECYT4 antibody | Endothelial PAS domain containing protein 1 antibody | Endotheli
Specifications & Purity
Validated, ExactAb™, 1.0 mg/mL
Biochemical and Physiological Mechanisms
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and
Host species
Rabbit
Specificity
HIF-2-alpha
Immunogen
A synthetic peptide derived from human EPAS-1 around the non-acetylation site of K385.
Positive Control
WB: HEK293, MCF7 and SH-SY5Y cell lysates. IHC: Human mammary cancer and colon tissues. ICC/IF: Human stomach and Mouse spleen tissues.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
HIF-2-alpha Antibody (Ab107509) - Western Blot All lanes: HIF-2-alpha Antibody (Ab107509) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 96 kDa Observed band size: 100 kDa Exposure time: 28.3 s
HIF-2-alpha Antibody (Ab107509) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HIF-2-alpha Antibody (Ab107509) was diluted at 1/100. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HIF-2-alpha Antibody (Ab107509) - IHC Immunohistochemistry analysis of paraffin-embedded Human mammary-cancer tissue using HIF-2-alpha Antibody (Ab107509) was diluted at 1/100. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of human-stomach tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of human-stomach tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of mouse-spleen tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of mouse-spleen tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/50-1/200
IHC
1/100-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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