Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A, 100×) - BioReagent, for Cell culture, Sterile

Cat. No.: I1523454

GRADE & PURITY BioReagent, for Cell culture, Sterile

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Overview

Insulin promotes the uptake of glucose and amino acids, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and also helps reduce the toxic levels of oxygen free radicals and peroxides. Selenium (supplied in the form of sodium selenite) acts as a cofactor for glutathione peroxidase and other proteins, and serves as an antioxidant in culture media. Sodium pyruvate is a precursor of amino acids, fatty acids and cholesterol, and is an important intermediate in many biosynthetic pathways. This product is intended for scientific research and production use, and is applied to the in vitro culture of tissues and cells.

Method of Use

Add to the culture medium at a final concentration of 1% (v/v).

Application

Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) is used as a basal medium supplement to reduce the dosage of fetal bovine serum (FBS) for cell culture. ITS-A solution is generally used in combination with 2–4% FBS.

Precautions

1. Thaw the product overnight at 4℃ before use.

2. Mix the product thoroughly before addition.

3. Strictly perform aseptic operation during use to avoid contamination.

4. Use the product within the shelf life; discard it once expired.

Specifications

Specifications & Purity

BioReagent, for Cell culture, Sterile

Stability And Storage

Store at -20℃ long term (24 months).

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Cell culture, Sterile