Articles by tag "Biological buffer"

1. Protocol of Western Blotting For High Molecular Weights
   posted this at Oct 29, 2024

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   Licia Miller   Product ManagerHigh molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.Required Solutions and Reagents (1) 10× Electrophoresis bufferStage 1 ...
2. Competent E. coli Cell Preparation Using the Calcium Chloride Method
   posted this at Sep 4, 2024

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   Product Manager：Harrison Michael1. Overview:The calcium chloride method is a well-established laboratory procedure used to prepare competent E. coli cells, which are capable of taking up foreign DNA through transformation. The process involves treating cells with a solution containing ...
3. Subcellular Fractionation Protocols
   posted this at Aug 20, 2024

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   Licia Miller   Product ManagerSubcellular fractionation is a biological experimental technique that allows researchers to separate and purify different components within cells, such as the nucleus, mitochondria, endoplasmic reticulum, etc., and then further study their structure and function. ...
4. Ethidium Bromide: Beautiful and Dangerous
   posted this at Aug 15, 2024

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   Product Manager：Harrison MichaelEthidium bromide (EtBr) is a classical fluorescent dye widely used in molecular biology research. Its chemical structure consists of a tri-benzimidazole, which can intercalate between the base pairs of DNA or RNA, significantly enhancing its fluorescence signal. ...
5. The Key to Gene Delivery to Cells —In Vitro Cell Transfection Techniques
   posted this at Jul 3, 2024

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   Product Manager：Harrison MichaelCell transfection techniques are the cornerstone of interdisciplinary research in molecular and cellular biology. They allow scientists to introduce foreign nucleic acids into cells to explore gene function, expression, and develop gene therapies or produce ...
6. Method and Precautions of SDS-PAGE Gel Preparation
   posted this at May 20, 2024

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   Product Manager：Harrison MichaelSDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the ...
7. Guidelines For Common Cell Culture Media
   posted this at May 15, 2024

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   Product Manager：Harrison MichaelCell culture medium is an indispensable part of cell biology research, which provides essential nutrients and a suitable develope environment for cells. The following is a comprehensive introduction to the detailed components and characteristics of several ...
8. Native ChIP (N-ChIP) Protocol
   posted this at Apr 10, 2024

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   Licia Miller   Product ManagerMaterials RequiredSolution recipes10 × TBS0.1 M Tris-HCl (pH 7.5)（example T301492） 1.5 M NaCl  30 mM CaCl2  20 mM MgCl2  50 mM Na butyrate (pH 8.0)  Digestion buffer0.32 M sucrose (example S112226) 50 mM Tris-HCl (pH 7.5) (example T301492) 4 mM ...
9. Immunocytochemistry (ICC) protocol
   posted this at Mar 17, 2024

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   Licia MillerProduct ManagerStage 1 - Sample preparation and fixationFixation of the sample is an essential step to maintain cell morphology during the ICC experiment and during storage.1, For cells cultured on coverslipsIn this procedure, cells are cultured directly on coverslips before ...