L-Glutamic Dehydrogenase (NADP), CAS No.9029-11-2

CAS: 9029-11-2 Cat. No.: L489276

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, >60 U/mg protein

Accession #

P00370

Bioactivity

>60 U/mg protein

Overview

Glutamate dehydrogenase (NADP) is the catalytic enzyme for glutamate production. Dysfunction of Glutamate dehydrogenase (NADP) may induce various neurodegenerative diseases, such as Parkinson's disease, epilepsy, Alzheimer's disease, schizophrenia, and frontotemporal dementia. Glutamate dehydrogenase (NADP) can be used in research on neurological diseases such as Parkinson's disease, epilepsy, and Alzheimer's disease.

Reaction Equation

L-Glutamate + H₂O + NADP⁺⇌ α-Ketoglutarate + NH₃ + NADPH + H⁺

Specification

Specific Activity: >60 U/mg protein (for reduction of α-Ketoglutarate to L-Glutamate)

Contaminants

Glucose 6-phosphate dehydrogenase: < 0.02%

Phosphogluconate dehydrogenase: < 0.1%

Glutamate dehydrogenase (NAD⁺): < 0.03%

Glutathione reductase: < 0.02%

NADPH oxidase: < 0.003%

Properties

pH stability: pH 5.0 - 10.5 (25 °C, 1 week)

Thermal stability: ≤70 °C (pH 7.5, 10 min)

Optimum pH: 7.5 - 8.0

Optimum temp.: ≥70 °C

Km value:

2.5 × 10² mol/L (L-Glutamate)

6.4 × 10⁵ mol/L (NADP⁺)

4.1 × 10⁴ mol/L (α-Ketoglutarate)

2.4 × 10⁵ mol/L (NADPH)

4.7 × 10⁵ mol/L (Ammonium acetate)

Molecular weight: 46 kDa (SDS-PAGE)

Assay Procedure

I Spectrophotometric Method

Wavelength: 340 nm, Light path length: 1 cm

Final volume: 3.02 mL, Temperature: 25 °C

Pipette the following reagents into a cuvette:

2.50 mL  Triethanolamine-HCl buffer (0.1 mol/L, pH 7.6)

0.15 mL  α-Ketoglutarate (0.1 mol/L)

0.05 mL  NADPH (12 mmol/L)

0.30 mL  Ammonium acetate (2 mol/L)

0.02 mL  rGIDH(NADP) (approx. 3 U/mL)

II Calculation

ΔA/min = The change in absorbance at 340 nm/minute

V = Total volume of reaction mixture (3.02 mL)

D = Enzyme dilution factor

6.2 = mmol/L extinction coefficient of NADPH (L·mmol⁻¹·cm⁻¹)

d = Light path length (1 cm)

v = Volume of enzyme sample (0.02 mL)

Specifications

Product Name

L-Glutamic Dehydrogenase (NADP), CAS No.9029-11-2

Synonyms

L-Glutamate:NADP+ oxidoreductase (deaminating) | Glutamate dehydrogenase (NADP⁺) | Glutamate Dehydrogenase (NADP-dependent) | glutamate dehydrogenase (NADP(+)) | glutamic dehydrogenase | GIDH (NADP) | gdhA

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, >60 U/mg protein

Biochemical and Physiological Mechanisms

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.

Bioactivity

>60 U/mg protein

Accession #

P00370

Source

bacterial

CAS

9029-11-2

Enzyme Commission Number

1.4.1.4

Molecule Type

Enzyme

Legal Information

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Storage and Shipping

Concentration

>60 U/mg protein

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Unit definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30 °C in the presence of ammonium ions and NADPH.

Images

L-谷氨酸脱氢酶（NADP型）

L-谷氨酸脱氢酶（NADP型）

L-谷氨酸脱氢酶（NADP型）

L-谷氨酸脱氢酶（NADP型）

L-谷氨酸脱氢酶（NADP型）