NECAB3 Antibody - Primary antibody, specific to NECAB3, Rabbit IgG

Cat. No.: Ab191500

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cow, Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human NECAB3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.

Specifications

Product Name

NECAB3 Antibody - Primary antibody, specific to NECAB3, Rabbit IgG

Synonyms

APBA2BP | NIP1 | SYTIP2 | XB51 | N-terminal EF-hand calcium-binding protein 3 | Amyloid beta A4 protein-binding family A member 2-binding protein | Nek2-interacting protein 1 | Neuronal calcium-binding protein 3 | X11L-binding protein 51 | NECAB3 | STIP3

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic conditions; the function requires its ABM domain and may

Host species

Rabbit

Specificity

NECAB3

Immunogen

A KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NIP1.

Positive Control

WB: COLO 205, HT-29 and Hep G2 cell lysates. IHC: Human lung cancer tissue. IF/ICC: COLO 205 cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: NECAB3 Antibody (Ab191500)

NECAB3 Antibody (Ab191500) - Western Blot
All lanes: NECAB3 Antibody (Ab191500) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 100.0 s

IHC: NECAB3 Antibody (Ab191500)

NECAB3 Antibody (Ab191500) - IHC
Immunohistochemical analysis of NECAB3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with NECAB3 Antibody (Ab191500) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: NECAB3 Antibody (Ab191500)

NECAB3 Antibody (Ab191500) - IF/ICC
Immunofluorescent analysis of NECAB3 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the NECAB3 Antibody (Ab191500) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Associated Targets(Human)

NECAB3 Tbio N-terminal EF-hand calcium-binding protein 3 (0 Activities)

Discover Target: NECAB3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)