GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Nitrate reductase (NR, EC 1.7.1.3) is widely present in plants. It is a key and inducible enzyme that catalyzes the conversion of nitrate nitrogen to ammonium nitrogen in plants, influencing crop yield and quality. This kit can detect nitrate reductase activity in plant tissues, bacterial or cellular samples. The principle is that NR catalyzes the reduction of nitrate to nitrite: NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O. The generated nitrite can quantitatively form a red azo compound with sulfanilic acid and α-naphthylamine under acidic conditions. This red azo compound exhibits a maximum absorption peak at 540 nm, allowing for detection by spectrophotometry.
Storage and Shipping
Storage
Protected from light,Store at -20°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for 6 months. Store in the dark.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.