GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human eIF3A Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/2000 ICC/IF: 1/50-1/200 Protein Function： Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Specifications
Product Name
Recombinant eIF3A Antibody - Primary antibody, specific to eIF3A, Rabbit IgG
A synthetic peptide derived from human eIF3A (AA 1100-1154)
Positive Control
WB: Jurkat, HeLa, Hep G2, HEK293, RAW 264.7, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant eIF3A Antibody (Ab101307) - Western Blot All lanes: Recombinant eIF3A Antibody (Ab101307) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 160 kDa Observed band size: 188 kDa Exposure time: 2.0 s
Recombinant eIF3A Antibody (Ab101307) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/50 dilution.
Recombinant eIF3A Antibody (Ab101307) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/50 dilution.
Recombinant eIF3A Antibody (Ab101307) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/150 dilution.
Recombinant eIF3A Antibody (Ab101307) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant eIF3A Antibody (Ab101307) at 1/200 dilution.
Application
Application
Dilution info
IF/ICC
1/50-1/200
WB
1/500-1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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