GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human HSPA12A Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/200
Specifications
Product Name
Recombinant HSPA12A Antibody - Primary antibody, specific to HSPA12A, Rabbit IgG
Synonyms
Heat shock 70 kDa protein 12A | FLJ13874 | heat shock 70kD protein 12A | heat shock 70kDa protein 12A | HS12A_HUMAN | Hspa12a | KIAA0417 | FLJ13874 antibody | Heat shock 70 kDa protein 12A antibody | heat shock 70kD protein 12A antibody | heat shock 70kDa
Specifications & Purity
Recombinant, ExactAb™, Validated, See COA
Host species
Rabbit
Specificity
HSPA12A
Immunogen
A synthetic peptide derived from human HSPA12A (AA 104-143)
Positive Control
WB: Mouse brain, HEK293 and Hep G2 lysates. IHC: Mouse brain tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant HSPA12A Antibody (Ab108950) - Western Blot All lanes: Recombinant HSPA12A Antibody (Ab108950) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 100.0 s
Recombinant HSPA12A Antibody (Ab108950) - Western Blot All lanes: Recombinant HSPA12A Antibody (Ab108950) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 34.7 s
Recombinant HSPA12A Antibody (Ab108950) - IHC Immunohistochemical analysis of paraffin-embedded mouse brain, using Recombinant HSPA12A Antibody (Ab108950).
Recombinant HSPA12A Antibody (Ab108950) - IF/ICC IF analysis of Recombinant HSPA12A Antibody (Ab108950) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HSPA12A Antibody (Ab108950) at 1/50 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IHC
1/50-1/200
WB
1/500-1/2000
IF/ICC
1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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