Recombinant Human CD40 Protein, >95%(SDS-PAGE)

Cat. No.: rp144000
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Fc tag ? Fc tag grade — recombinant protein bearing a Fc tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. The Fc tag aids dimerization and protein-A/G capture. ≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P25942
Protein Tag
C-hFc
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized human CD40L-His at 10 μg/mL (100 μL/well) can bind Human CD40-Fc. The EC50 of Human CD40-Fc is 10-30 ng/mL.
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
rp144000-10μg
10
$139.90
50μg
rp144000-50μg
4
$379.90
100μg
rp144000-100μg
1
$559.90
1mg
rp144000-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,429.90
Enter a quantity for the sizes you want to add.
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Why this grade

Carrier Free,Bioactive,ActiBioPure™,Azide Free,Fc tag,≥95%(SDS-PAGE) ActiBioPure™,Azide Free,Bioactive,Carrier Free,Fc tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Purity
>95% SDS-PAGE.

Function
Receptor for TNFSF5/CD40LG.

Specifications

Product Name
Recombinant Human CD40 Protein, >95%(SDS-PAGE)
Synonyms
AI326936 | B cell associated molecule CD40 | B cell surface antigen CD40 | B cell-associated molecule | B-cell surface antigen CD40 | Bp50 | CD 40 | CD40 | CD40 antigen | CD40 antigen (TNF receptor superfamily member 5) | CD40 molecule | CD40 molecule, TN
Grade
ActiBioPure™, Azide Free, Bioactive, Carrier Free, Fc tag
Specifications & Purity
Carrier Free, Bioactive, ActiBioPure™, Azide Free, Fc tag, ≥95%(SDS-PAGE)
Purity
>95%(SDS-PAGE)
Bioactivity
Immobilized human CD40L-His at 10 μg/mL (100 μL/well) can bind Human CD40-Fc. The EC50 of Human CD40-Fc is 10-30 ng/mL.
Endotoxin Concentration
<1.0 EU/μg
Expression System
HEK293
Species
Human
Amino Acids
21-193 aa
Sequence
EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLRALEVLFQGPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Protein Tag
C-Fc
Accession #
Predicted molecular weight
46.2 kDa
SDS-PAGE
54.1 kDa, reducing conditions
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
It is recommended that sterile water (1100ul) be added to the vial to prepare a stock solution of 0.91mg/mL.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Samples are stable for twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Images

Recombinant Human CD40 Protein (rp144000) - Protein Bioactivity
Immobilized Recombinant Human CD40L/CD154/TNFSF5 Protein at 2.0 μg/mL can bind Recombinant Human CD40 Protein (rp144000), the EC₅₀ is 10 - 30 ng/mL.

Recombinant Human CD40 Protein (rp144000) - SDS-PAGE
3 μg/lane of Recombinant Human CD40 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 57.5 kDa under reducing conditions and 113.9 kDa under non-reducing conditions.

Recombinant Human CD40 Protein (rp144000) - ELISA
Immobilized Recombinant Human CD40 Protein (rp144000) at 1.0 μg/mL can bind Teneliximab (anti-CD40) (Ab183440) with the EC50 of 11.50 ng/mL.​

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505380Certificate of AnalysisMay 15, 2024 rp144000
ZJ24F0505379Certificate of AnalysisMay 15, 2024 rp144000
ZJ24F0505378Certificate of AnalysisMay 15, 2024 rp144000
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

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