GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)See COA
Accession #
O75874-1
Protein Tag
C-His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
1、Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions. 2、Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC50 of 11.16 ng/mL.
Carrier Free,Bioactive,ActiBioPure™,His-Tag,≥95%(SDS-PAGE),See COA ActiBioPure™,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Specifications
Product Name
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein
Carrier Free, Bioactive, ActiBioPure™, His-Tag, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Isocitrate Dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate to alpha ‑ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+. The protein encoded
Bioactivity
1、Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10, 000 pmol/min/μg, as measured under the described conditions. 2、Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC50 of 11.16 ng/mL.
44.2 kDa, under reducing conditions; 43.6 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Concentration
See COA
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Images
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) - SDS-PAGE 3 μg/lane of Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 44.2 kDa under reducing conditions and 43.6 kDa under non-reducing conditions.
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein - Enzyme Activity Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions. Materials: Assay Buffer: 25 mM Tris, 0.5 mM MnCl₂, 5 mM DTT, pH 7.5 Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) DL-Isocitric acid (I106830), 100 mM stock in deionized water NADP⁺ (N303921), 50 mM stock in deionized water
Procedures： 1. Dilute rhIDH1 to 0.4 ng/μL in Assay Buffer. 2. Prepare a Substrate Mixture by Diluting NADP
⁺
and Isocitric Acid to 1 mM and 2 mM, respectively, in Assay Buffer. 3. Load into a plate 50 μL of 0.4 ng/μL rhIDH1 and start the reaction by adding 50 μL of Substrate Mix. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of Substrate Mix. 4. Read plate at a wavelength of 340 nm (bottom read) in kinetic mode for 5 minutes. 5. Calculate specific activity: *Adjusted for Substrate Blank **Using the extinction coefficient 6270 M⁻¹cm⁻¹ ***Using the path correction 0.32 cm Per Well: rhIDH1: 0.020 μg, NADP⁺
: 0.5 mM, Isocitric Acid: 1 mM
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein - ELISA Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC₅₀ of 11.16 ng/mL.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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*Adjusted for Substrate Blank
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