Recombinant Human PTEN Protein

Cat. No.: rp183747

GRADE & PURITY Carrier Free, Azide Free, His Tag, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

P60484

Protein Tag

C-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Overview

Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities. Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol-triphosphate specifically at position 3 of the inositol ring. This activity reduces the levels of phosphatidylinositol-triphosphate which is specifically produced from phosphatidylinositol-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity.

Specifications

Product Name

Recombinant Human PTEN Protein

Synonyms

10q23del | BZS | DEC | GLM2 | MGC11227 | MHAM | MMAC1 | MMAC1 phosphatase and tensin homolog deleted on chromosome 10 | Mutated in multiple advanced cancers 1 | Phosphatase and tensin homolog | Phosphatase and tensin like protein | Phosphatidylinositol 3,

Grade

Azide Free, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Azide Free, His-Tag, ≥90%(SDS-PAGE)

Endotoxin Concentration

<1.0 EU/μg

Expression System

E. coli

Amino Acids

1-403 aa

Sequence

MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKVHHHHHH

Protein Tag

C-His

Accession #

P60484

Predicted molecular weight

47.9 kDa

SDS-PAGE

52.8 kDa, under reducing conditions; 51.9 kDa, under non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human PTEN Protein (rp183747)

Recombinant Human PTEN Protein (rp183747) - SDS-PAGE
3 μg/lane of Recombinant Human PTEN Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 52.8 kDa under reducing conditions and 51.9 kDa under non-reducing conditions.