Recombinant MHC Class II Antibody - Primary antibody, specific to MHC Class II

Cat. No.: Ab212163
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
ELISA, Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2b
Clone Number
P24J10
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab212163-10μg
10
$19.90
50μg
Ab212163-50μg
7
$53.90
100μg
Ab212163-100μg
4
$85.90
1mg
Ab212163-1mg
1
$479.90
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free,Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),See COA Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant MHC Class II Antibody, clone P24J10, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name
Recombinant MHC Class II Antibody - Primary antibody, specific to MHC Class II
Synonyms
D6S221E antibody | DMA antibody | DMB antibody | DP beta 1 antibody | DP beta 1 chain antibody | DP(W4) beta chain antibody | DPB 1 antibody | DPB1 antibody | DPB1_HUMAN antibody | DRB antibody | H2Ea antibody | HLA class II histocompatibility antigen ant
Specifications & Purity
Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides
Host species
Rat
Specificity
MHC Class II
Immunogen
Activated C57BL/6 mouse spleen cells
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
Unconjugated
Grade
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

Recombinant MHC Class II Antibody (Ab212163) - Flow Cytometry
Flow Cytometry analysis of C57BL/6 mouse splenocytes labelling MHC Class II  with Recombinant MHC Class II Antibody (Ab212163) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Recombinant MHC Class II Antibody (Ab212163) - Flow Cytometry
Flow Cytometry analysis of C57BL/6 mouse splenocytes labelling MHC Class II (red) with Recombinant MHC Class II Antibody (Ab212163) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Recombinant MHC Class II Antibody (Ab212163) - SEC
The purity of Recombinant MHC Class II Antibody (Ab212163) is more than 95% verified by HPLC.

Application
ApplicationDilution info
Flow Cytometry

≤0.5 µg per million cells in 100 µl volume

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
HLA-DPB1 Tbio HLA class II histocompatibility antigen, DP beta 1 chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

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