Tetramethylrhodamine - ≥98% , CAS No.115532-49-5

CAS: 115532-49-5 Cat. No.: T275831 Molecular Weight: 401.48 PubChem CID: 5009757

GRADE & PURITY ≥98%

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Overview

Shipped at 4°C. Store at +4°C. Store In the Dark. Store under desiccating conditions.

Specifications

Specifications & Purity

≥98%

Biochemical and Physiological Mechanisms

Fluorescent, cell-permeant cationic dye for measuring mitochondrial membrane potential. Accumulates in healthy, negatively charged mitochondria. Exhibits re-orange fluorscence (Em = 573 nm).

Storage

Protected from light, Store at -20°C, Desiccated

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Refer to SDS for further information Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥98%

Names and Identifiers

• Pubchem Sid: 488195058
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488195058
• Canonical Smiles: CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=CC=CC=C4C(=O)OC
• IUPAC Name: [6-(dimethylamino)-9-(2-methoxycarbonylphenyl)xanthen-3-ylidene]-dimethylazanium
• InChIKey: WAWRKBQQBUDAMY-UHFFFAOYSA-N
• INCHI: 1S/C25H25N2O3/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5/h6-15H,1-5H3/q+1
• Isomeric SMILES: CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=CC=CC=C4C(=O)OC
• PubChem CID: 5009757
• Molecular Weight: 401.48

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Benzoic acid esters Dialkylarylamines Benzoyl derivatives Secondary ketimines Methyl esters Heteroaromatic compounds Amino acids and derivatives Oxacyclic compounds Organooxygen compounds Organic oxides Hydrocarbon derivatives Organic cations
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Benzoate ester - Benzoic acid or derivatives - Benzoyl - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Benzenoid - Monocyclic benzene moiety - Heteroaromatic compound - Methyl ester - Secondary ketimine - Tertiary amine - Amino acid or derivatives - Carboxylic acid ester - Oxacycle - Carboxylic acid derivative - Hydrocarbon derivative - Organic oxide - Organonitrogen compound - Organooxygen compound - Amine - Organic oxygen compound - Organic nitrogen compound - Organic cation - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Soluble in DMSO and in ethanol
• Molecular Weight: 401.500 g/mol
• XLogP3: 3.300
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 4
• Exact Mass: 401.187 Da
• Monoisotopic Mass: 401.187 Da
• Topological Polar Surface Area: 41.800 Ų
• Heavy Atom Count: 30
• Formal Charge: 1
• Complexity: 772.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Xindong Wang, Xinyu Wang, Bo Qu, Nuernisha Alifu, Ji Qi, Ruiyuan Liu, Qinrui Fu, Ruifang Shen, Qi Xia, Lijun Wu, Bing Sun, Jibin Song, Youping Lin, Xin Huang, Anjun Qin, Jun Qian, Ben Zhong Tang, Guanying Chen.  (2021)  A Class of Biocompatible Dye–Protein Complex Optical Nanoprobes.  ACS Nano,      [PMID:34939417] [10.1021/acsnano.1c06536]

2. Ye Yuan, Changyong Gao, Zhexu Wang, Jianming Fan, Haofei Zhou, Daolin Wang, Chang Zhou, Baohua Zhu, Qiang He.  (2021)  Upconversion-nanoparticle-functionalized Janus micromotors for efficient detection of uric acid.  Journal of Materials Chemistry B,  10  (3): (358-363).  [PMID:35005767] [10.1039/D1TB02550C]

3. Yicheng Lv, Xinyi Zhou, Mingyue Wang, Mengyao Qi, Wenqi Xie, Chuang Liu, Zixuan Dong, Xiaoling Fu.  (2025)  Mitochondria-Loaded Janus Cardiac Patch for Myocardial Infarction Repair and Prevention of Tissue Adhesions.  ACS Nano,      [PMID:41432052] [10.1021/acsnano.5c18334]

4. Wei Li, Xiao Wang, Xiaoli Liu, Luoyang Wang, Jie Liang, Yu Cui, Yi Liu, Mengting Zhou, Zijie Yang, Meiying Song, Yanyan Yang, Li Zhang, Bei Zhang.  (2026)  Lactate drive M2 polarization via OXPHOS and Cebpb and accelerate peripheral nerve regeneration.  INTERNATIONAL IMMUNOPHARMACOLOGY,      [PMID:] [10.1016/j.intimp.2026.116474]