FAQs

If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?

· Reviewed · Published Jun 9, 2025 · Updated Aug 27, 2024 · 8 min read

If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?

If the protein sample contains a relatively large number of dimers, the reducing agent DTT can be added to help open the dimers.

Categories: FAQs

Explore topics: Buffer protein Polymer heterobands Dimers

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?" Aladdin Knowledge Base, updated Aug 27, 2024. https://www.aladdinsci.com/us_en/faqs/i-need-to-add-doubling-buffer-to-open-the-dimers-en.html

Was this article helpful? Yes No 1 out 2 found this helpful