Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The core of protein chromatographic purification is to maintain the target protein in a conformationally stable state under operating conditions, while enabling controllable interactions and reproducible separation behavior. Buffers do not only define pH; through temperature coefficients, ionic ...
The streptavidin–biotin system has extremely high affinity and is one of the most commonly used tool pairs for immobilization, capture, and elution of nucleic acids and proteins.
Maintaining a stable extracellular pH minimizes nonspecific fluctuations in metabolism and signaling.
Antibodies, as core molecules in biopharmaceuticals, play a crucial role in determining both product quality and production costs. Tag technologies, as key tools in antibody purification, have evolved from mature applications in classical systems to innovative breakthroughs in emerging systems, ...
Polymerase Chain Reaction (PCR) is a core technology in molecular biology, clinical diagnostics, and scientific research. Its high sensitivity means that even minute contaminants can affect experimental results, leading to false positives or false negatives.
Can tumor tissue cuts be stored for a long time after fixation in 4% paraformaldehyde fixative at room temperature?
What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
Does the protein Buffer contain denaturants?
BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
If the concentration of the denatured sample is high, how should it be diluted?
If the protein contains polymer heterobands, dimers may be present, do I need to add doubling buffer to open the dimers?
After adding buffer to boil denatured proteins for cryopreservation, do they still need to be boiled after they are removed again? Why?
Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
Does the protein's own charge affect the buffer?
Is there a requirement for protein concentration for this product? If the protein concentration is high, do I need to increase the concentration of the buffer?
What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature. Regulatory or purity needs for your exact application should also be considered. The following tables can help you navigate preparation of many ...
Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
Polyethylene glycol (PEG) linker is a chemical sensing the carrier of polyethylene glycol (PEG), due to its solubility in water and non-immunogenicity. In the field of scientific research, it is widely used in chemical coupling, drug delivery, nanoparticles functionalized modifications and ...
Techniques often explored alongside immunological experiments.
