The core of protein chromatographic purification is to maintain the target protein in a conformationally stable state under operating conditions, while enabling controllable interactions and reproducible separation behavior. Buffers do not only define pH; through temperature coefficients, ionic ...
The streptavidin–biotin system has extremely high affinity and is one of the most commonly used tool pairs for immobilization, capture, and elution of nucleic acids and proteins.
Antibodies, as core molecules in biopharmaceuticals, play a crucial role in determining both product quality and production costs. Tag technologies, as key tools in antibody purification, have evolved from mature applications in classical systems to innovative breakthroughs in emerging systems, ...
Polymerase Chain Reaction (PCR) is a core technology in molecular biology, clinical diagnostics, and scientific research. Its high sensitivity means that even minute contaminants can affect experimental results, leading to false positives or false negatives.
What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
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