Measurement of neutrophil phagocytosis
Measurement of neutrophil phagocytosis
Measurement of neutrophil phagocytosis can be used to (1) detect phagocytosis in the body and (2) diagnose diseases associated with phagocyte dysfunction.
Operation method
Measurement of neutrophil phagocytosis
Principle
Neutrophils are the most abundant immune cells in the peripheral blood circulation and the immune system, with deformation and phagocytosis, and play a very important role in the fight against disease and protection of the body. Neutrophils can adhere to the surface of large venous endothelial cells and can be rapidly mobilized in response to infection and stress. They have a variety of biological functions such as chemotaxis, phagocytosis and sterilization, and are an important part of the body's intrinsic immune system. After mixing leukocyte suspension with bacteria for a period of time, the sample is taken and stained for microscopic examination, which can be used to determine the phagocytosis percentage and phagocytosis index of neutrophils, and to judge the phagocytosis function of neutrophils.
Materials and Instruments
Staphylococcus aureus (suspension) Move I. Method of operation 1. Take 1 small test tube and add 1 drop of 3.8% sodium citrate solution (0.4 ml). 2. Sterilize the finger and blood collection needle with alcohol cotton wool, take 2-3 drops of blood from the sterilized site (about 0.2 ml) and add it to the small test tube, mix well. 3. Take 1 drop (about 0.1 ml) of bacterial solution and add it into a small test tube, blow and mix well. 4. Place in a 37℃ water bath for 15 min, and mix once halfway. 5. Remove the small test tube, use a pipette to beat the mixed blood in the test tube, take half a drop of blood on a slide, and use another slide to push into a thin slice of blood. 6. The slide was allowed to stand, and the blood was allowed to dry on its own, and then stained with Rachel's stain. II. Analysis of results Caveat 1. There are differences in phagocytosis due to individual differences in different specimens as well as different ages and health conditions. 2. The blood smear should not be too thick, otherwise it is not easy to observe the results. 3. This experiment belongs to live bacteria experiment, pay attention to aseptic operation and individual biosafety. For more product details, please visit Aladdin Scientific website.
Broth medium Alkaline melphalan solution Physiological saline Methanol Anticoagulant (3.8% sodium citrate)
Incubator Water bath Hemoglobin pipette Slide Small test tube Slide Blood collection needle Alcohol cotton ball
Phagocytosis % = i.e. the number of cells with bacteria phagocytosed in 100 neutrophils;
Phagocytosis index = divide the total number of bacteria phagocytosed by 100 neutrophils by 100 to get the average number of bacteria phagocytosed by each leukocyte, which is the phagocytosis index.