GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Alkaline Phosphatase Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant Alkaline Phosphatase Antibody - Primary antibody, specific to Alkaline Phosphatase, Rabbit IgG
Recombinant, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particula
Host species
Rabbit
Specificity
Alkaline Phosphatase
Immunogen
A synthetic peptide derived from human Alkaline Phosphatase (AA 1-61).
Positive Control
WB: Mouse kidney, Rat kidney, HeLa, Hep G2 and JAR lysates. IHC: Human liver tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Alkaline Phosphatase Antibody (Ab088188) - Western Blot All lanes: Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 60.0 s
Recombinant Alkaline Phosphatase Antibody (Ab088188) - Western Blot All lanes: Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 30.9 s
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188).
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/50 dilution.
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/150 dilution.
Recombinant Alkaline Phosphatase Antibody (Ab088188) - IHC Immunohistochemical analysis of paraffin-embedded human liver, using Recombinant Alkaline Phosphatase Antibody (Ab088188).
IF analysis of Recombinant Alkaline Phosphatase Antibody (Ab088188) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Alkaline Phosphatase Antibody (Ab088188) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IF/ICC
1/50-1/200
IHC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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