GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human GATA4 Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Specifications
Product Name
Recombinant GATA4 Antibody - Primary antibody, specific to GATA4, Rabbit IgG
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity).
Host species
Rabbit
Specificity
GATA4
Immunogen
A synthetic peptide derived from human GATA4 (AA 5-48)
Positive Control
WB: Hep G2, HEK293 and HeLa cell lysates. IF/ICC: Hep G2 cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant GATA4 Antibody (Ab104893) - Western Blot All lanes: Recombinant GATA4 Antibody (Ab104893) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution Predicted band size: 45 kDa Observed band size: 22, 57 kDa ~20 kDa band is consistent with what has been described in the literature (PMID: 25501827). Caspase-1 mediated cleavage of GATA4 generates a truncated protein that retains the ability to bind DNA but lacks transcriptional activation domains and acts as a dominant negative regulator of GATA4. Exposure time: 167.7 s
Recombinant GATA4 Antibody (Ab104893) - IF/ICC Immunofluorescent analysis of Hep G2 cells, using Recombinant GATA4 Antibody (Ab104893).
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/50 - 1/200
ICC/IF
1/100-1/500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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