Recombinant Human B7-1/CD80 Protein, ≥95% (SDS-PAGE)

Cat. No.: rp144120
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. His Tag ? His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. ≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P33681
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
rp144120-10μg
7
$77.90
50μg
rp144120-50μg
3
$241.90
100μg
rp144120-100μg
1
$419.90
1mg
rp144120-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,899.90
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,His-Tag,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Specifications

Product Name
Recombinant Human B7-1/CD80 Protein, ≥95% (SDS-PAGE)
Synonyms
Activation B7-1 antigen | B7 | BB1 | CTLA-4 counter-receptor B7.1 | Activation B7-1 antigen | B lymphocyte activation antigen B7 | B7 | B7-1 | B7-1 antigen | B7.1 | BB1 | CD28 antigen ligand 1 | CD28LG | CD28LG1 | CD80 | CD80 antigen | CD80 antigen (CD28
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, His Tag
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥95%(SDS-PAGE)
Purity
≥95% (SDS-PAGE)
Bioactivity
Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.
Endotoxin Concentration
<0.1 EU/μg
Expression System
HEK293
Species
Human
Amino Acids
35-242 aa
Sequence
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNHHHHHH
Protein Tag
C-His
Accession #
Predicted molecular weight
25 kDa
SDS-PAGE
40 - 55 kDa, under reducing conditions; 40 - 55 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Shape
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant Human B7-1/CD80 Protein (rp144120) - Protein Bioactivity
Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.

Recombinant Human B7-1/CD80 Protein (rp144120) - SDS-PAGE
3 μg/lane of Recombinant Human B7-1/CD80 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40 - 55 kDa.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

6 results found

Lot NumberCertificate TypeDateItem
ZJ24F1215478Certificate of AnalysisJul 10, 2026 rp144120
ZJ24F1215479Certificate of AnalysisJul 10, 2026 rp144120
ZJ24F1215480Certificate of AnalysisJul 10, 2026 rp144120
ZJ24F0809591Certificate of AnalysisMar 13, 2026 rp144120
ZJ24F0809592Certificate of AnalysisMar 13, 2026 rp144120
ZJ24F0809593Certificate of AnalysisMar 13, 2026 rp144120
Documents & Articles
Solution Calculators
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