GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥50U/mg enzyme powder; ≥200U/mg protein
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥50U/mg enzyme powder; ≥200U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Malate dehydrogenase (MDH) catalyzes the mutual conversion of oxaloacetate and malate, and is associated with the oxidation/reduction of dinucleotide coenzymes.
Reaction
Product description
Appearance:
White amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 1.1.1.37
CAS Number:
9001-64-3
Storage temperature:
-20℃
Activity:
≥50U/mg enzyme powder; ≥200U/mg protein
Unit definition:
One unit will convert one micromole of oxaloacetate to L-malate per min at pH 7.5 at 30℃.
Properties
Stability:
Stable at -20℃ for at least two years
Molecular weight:
34 kDa (SDS-PAGE)
Isoelectric point:
4.9
Michaelis constant:
2.5×10⁻⁵M (NADH)2.0×10⁻⁵M (Oxaloacetate)
Optimum pH:
9.0
{Fig. 1}
Optimum temperature:
55℃
{Fig. 3}
pH Stability:
3.5-8.5 (25℃, 20hr)
{Fig. 2}
Thermal stability:
< 60℃ (pH7.5, 15min)
{Fig. 4}
Inhibitors:
Cu²⁺,Ni²⁺,SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on MDH
[The enzyme dissolved in 100mM Tris-HCl buffer, pH 7.5 (17U/ml) was incubated with each chemical at 37℃ for 2hr.]
Specifications
Product Name
Recombinant Malate Dehydrogenase (MDH), CAS No.9001-64-3
Synonyms
L-Malate: NAD+ oxidoreductase | Malic dehydrogenase | mdh | ECK3225
Grade
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥50U/mg enzyme powder; ≥200U/mg protein
Biochemical and Physiological Mechanisms
Catalyzes the reversible oxidation of malate to oxaloacetate.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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